Nervous Tissue Pre-Lab 1
Nervous tissue is a difficult tissue to study with a single method. Masses of nerve fibres in the CNS appear as an almost homogeneous material with H&E staining - indeed, they used to be called neuropil ("nerve-felt") once. Methods used to demonstrate fibres in the CNS usually do not show neuron bodies to advantage. In these pages, images taken with a variety of techniques have been used, all of which may not be in your slide box.
In this lab, we are limiting ourselves to being able to recognise some basic features of neurons. We begin with a section of the spinal cord.
microscope, recognise the central, H-shaped grey matter.
line drawn across the picture is the boundary between the grey and white
matter. Five large neuron bodies are obvious here.
Try this :
Remember : With large neurons it is easy to mistake the large nucleus for the cell and the nucleolus for the nucleus! Do not fall into this trap!